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Image Search Results
Journal: bioRxiv
Article Title: Novel strategies of Raman imaging for monitoring intracellular retinoid metabolism in cancer cells
doi: 10.1101/2020.05.05.078410
Figure Lengend Snippet: Microscopy images of lipid droplets stained with Oil Red O dye in normal human astrocytes NHA (A) and high-grade glioblastoma U-87 MG (B and L) with bar plots of the total number of lipid droplets in NHA and U-87 MG , the area of LD per cell and the size of LDs were quantified by Image J software . The results represent the means +/- SEM of at least 6 representative cells (n(NHA)=6, n(U-87 MG)=9), Raman spectra of lipid droplets of normal astrocytes, profile I – TAG (orange), profile II – retinyl esters (blue) (C), microscopy image (D), Raman cluster image (E)(image size 50×50 μm, resolution 1 μm, integration time 0.3 second, number of Raman spectra 2500) and Raman image of the distribution of lipid droplets with profile I (F) and profile II (G) in NHA. Average Raman spectrum of lipid droplets obtained from cluster analysis (H), microscopy image (I), Raman cluster image (J)(image size 55×20 μm, resolution 1 μm, integration time 0.3 second, number of Raman spectra 1100) and fluorescence image of Oil Red O (K) and microscopy images of Oil Red O-stained lipid droplets (L) of U-87 MG high-grade glioblastoma. Raman analysis were performed at 532 nm laser excitation. Nucleus was labeled by red, lipid droplets – blue/orange, cytoplasm - green, mitochondria - magenta, cell border - light grey and area out of cell - dark grey.
Article Snippet: A normal
Techniques: Microscopy, Staining, Software, Fluorescence, Labeling